Fig. 5. Pra-B inhibits TPA-induced migration and invasion through inactivation of the AKT pathway. HeLa cells were cotreated with TPA and Pra-B in the absence or presence of LY294002 for 24 h. (A) The expression of p-AKT, AKT, MMP-2, and MMP-9 was measured through Western blotting. β-actin was used as an internal control. (B) Cell migration and invasion were measured using the Boyden chamber with polycarbonate filters. (C) MMP-2/-9 promoter activity levels were measured using the luciferase assay. These results are representative of three independent experiments. The values shown are the mean ± SE. **P<0.01 compared with untreated cells. #P<0.01 compared with TPA alone.